- $3 million gift to the University of Pittsburgh Cancer Institute to establish the Mario Lemieux Center for Blood Cancers
- $1.6 million gift in 2008 to Children’s Hospital of Pittsburgh of UPMC for Pediatric Hematology/Oncology Research and the establishment of two playrooms – The Lemieux Sibling Center in the main lobby and an Austin’s Playroom on the sixth floor for in-patients
- $2 million gift to The Children’s Home of Pittsburgh to create the Lemieux Family Center
- $1 million gift in 2002 to Children’s Hospital of Pittsburgh of UPMC for Pediatric Cancer Research
- $5 million gift in 2001 to UPMC Health System to establish the Mario Lemieux Centers for Patient Care and Research
